中国免疫学杂志2012，Vol.28Issue(2)：122-124,131,4.DOI:10.3969/j.issn.1000-484X.2012.02.006
癌干细胞标记物CD44与ESA在舌癌淋巴道转移模型中的表达及其意义
Expressions and significance of cancer stem cell markers CD44 and ESA in the metastatic tongue carcinoma lymph nodes of mice
摘要
Abstract
Objective: To explore the expressions and significance of CSCs associated markers CD44 and ESA in the metastatic tongue carcinoma lymph nodes of mice. Methods: Lymphatic metastatic animal model of tongue carcinoma through foot pad injection of cancer cells was established and metastatic lymph nodes were collected. Cell cultivating technique was employed to induce metastasis in lymph node and to obtain immortalized tongue carcinoma cell line from the metastatic cell. The immortalllized tongue carcinoma cell line cells were subsequently injected into nude mice via the foot pad. The cancer stem cell associated markers,such as CD44 and ESA, were detected by immunohistochemical staining from specimens of metastatic lymph node 4 weeks after injection. Results: In the lymphatic metastatic tongue carcinoma cells, staining of CD44 and ESA were positive. Conclusion: In the lymphatic metastatic tongue carcinoma cells, staining of CD44 and ESA of the concerned surface markers of cancer stem cells were positive, which shows that there may exist CSCs in the metastatic lymph nodes,and hints that the ' seeds' of cancer metastasis may be CSCs.关键词
癌干细胞/舌癌/CD44/ESA/免疫组织化学技术Key words
Cancer stem cells/Tongue carcinoma/CD44/ESA/Immunohistochemistry分类
医药卫生引用本文复制引用
邹维艳,王俊斌,严海芹,孙美群,邝晓聪..癌干细胞标记物CD44与ESA在舌癌淋巴道转移模型中的表达及其意义[J].中国免疫学杂志,2012,28(2):122-124,131,4.基金项目
本文受省高等学校优秀青年人才基金项目(No.2011SQRL082)和院级课题项目(No.by1002)资助 （No.2011SQRL082）
