中国免疫学杂志2012，Vol.28Issue(2)：155-160,6.DOI:10.3969/j.issn.1000-484X.2012.02.013
新生大鼠缺氧缺血性脑损伤tPA、PAI-1表达的动态变化
Expressions of tPA and PAI-1 in neonatal rats with hypoxic-ischemic brain damage
郭世杰 1刘英 1韩军 1周文莉1
作者信息
- 1. 吉林大学第一医院儿科,长春,130021
- 折叠
摘要
Abstract
Objective; To observe the expressions of tissue-type plasminogen activator( tPA ) and plasminogen activator inhibi-tor-lC PAI-1 ) in neonatal rats with hypoxic-ischemic brain damage( HIBD ) and the effect of fibrinolytic system in HIBD. Methods :To-tally 96 seven-day-old SD rat pups were randomly assigned into 2 groups; sham operation group and HIBD group. Rat pups were decollated to extract brain tissue at different time points ( 3,6,12,24,36,48 ,72,96 hours ) after animal models were made. The expressions of tPA and PAI-1 in rat pups were detected by immunohistochemistry and in situ hybridization methods. Results ;The protein and mRNA of tPA and PAI-1 were slightly expressed in all parts of brain with sham operation group. The protein and mRNA of tPA and PAI-1 were expressed in different time points with HIBD group; tPA was expressed at 3 hours, mainly in cortex and hippocampus. It was obviously in neurons, but slightly in endothelium of capillary vessel. It was increased gradually in neurons and endothelium of capillary vessel at 48 hours. It was decreased in neurons, but still increased in endothelium of capillary vessel at 72 hours. It was decreased gradually after 72 hours. The quantities of positive neurons in HIBD group were more than sham operation group from 3 hours to 96 hours. The protein and mRNA of PAI-1 were expreesed at 12 hours. It was increased gradually in neurons and endothelium of capillary vessel, and reached to the peak at 72 hours. The quantities of positive neurons in HIBD group were more than sham operation group from 12 hours to 96 hours. Conclusion :tPA and PAI-1 participate the pathogenesis of HIBD.关键词
缺氧缺血性脑损伤/组织型纤溶酶原激活物(tPA)/1型纤溶酶原激活物抑制剂(PAI-1)/免疫组织化学/原位杂交Key words
Hypoxic-ischemic brain damage/Tissue-type plasminogen activator/Plasminogen activator inhibitor-1/ Immunohis-tochemistry/In situ hybridization分类
医药卫生引用本文复制引用
郭世杰,刘英,韩军,周文莉..新生大鼠缺氧缺血性脑损伤tPA、PAI-1表达的动态变化[J].中国免疫学杂志,2012,28(2):155-160,6.
