中国农业大学学报2011,Vol.16Issue(5):50-54,5.
丁香属植物亲缘关系的AFLP分析
Analysis of the phylogenetic relationship of Syringa by AFLP technique
摘要
Abstract
To explore the relationships among Syring vulgaris, species of Syringa, Syringa and Ligustrum, eight EcoR I /Mse I primer combinations were used for AFLP molecular marker technique. The genetic diversity of 14 Syringa species,37 cultivars of S. vulgaris and 3 Ligustrum species were evaluated. NTSYS was used to compute the genetic distance and UPGMA for clustering analysis. The results showed that about 447 discernable bands were obtained,395 being polymorphic with the percentage of 88.4%. The genetic distance computed by NTSYS was from 0.01 to 1.44. There were some differences between the clustering analysis and traditional taxonomic method, in which some Syring vulgaris were clusted with Ser. villosae, sect Ligustrina with Ser. syringa, species in Ser. villosae and Ser. pubescente,the Ligustrum species of S. pinnatifolia and S. microphylla. It implied that Ser. villosae participated the breeding process of some Syringa vulgaris. The classification of Syringa should break into two sects divide. Ser. villosae had a close relationship with Ser. pubescente. Syringa had a complex phylogenetic relationship. The further study is required for the origin and classification relationship of Syringa and Ligustrum.关键词
丁香种/欧丁香品种/AFLP/多样性Key words
Syringa species/cultivars of S. vulgaris/AFLP/diversity分类
农业科技引用本文复制引用
高洪晓,杨凯,刘建斌..丁香属植物亲缘关系的AFLP分析[J].中国农业大学学报,2011,16(5):50-54,5.基金项目
北京市教委科技计划面上项目(KM200510020003) ()