中国农业大学学报2011，Vol.16Issue(5)：88-95,8.
芦丁对哺乳大鼠乳腺发育及相关激素水平与受体表达量关系的影响
Effect of dietary rutin on mammary gland development and the level and receptors of estrogen in lactation rats
摘要
Abstract
The objective of this study was to observe effects of a natural plant extract rutin in mammary gland development,endocrine hormone levels and receptor expression were measured for lactation rats. Eighteen Wistar rats were randomly assigned to three treatments,control （Lac-Con） group was treated with gastric infusion of 2 mL normal saline per mouse per day Rutin （Lac-Rut） treatment group was treated with gastric infusion of rutin 60 mg/kg BW per day,and Estradiol （Lac-Est） group was treated with intramuscular injection of estradiol 60μg/kg BW weekly started from the fourth lactation day and continue for two weeks with the same basal diet as control group. The level of estrogen （E2） ,progesterone （P） ,proiactin （PRL） and growth hormone （GH） in plasma and gland tissues were detected and the expression of estrogen receptor （ER）, progesterone receptor （PR）, prolactin receptor （PRLR） and growth hormone receptor （GHR） in gland tissues were measured. Histomorphology of development about mammary gland were observed. （Results）The results showed that, the relationship among the level of E2, PRL and GH in plasma and gland tissues was Lac-Est〉Lac-Rut~Lac-Con （P〈0.05）, the ）evel of P in plasma and gland tissues was not significant among groups （P〉0. 05）. The expression of ER, PR, PRLR and GHR in gland tissues was Lac-Est〉 Lac-Rut〉 Lac-Con （P〈0.05）. The diameter of mammary gland alveolus showed that Lac-Est and Lac-Rut were higher than Lac-Con （P〈 0.05）, there was not significant between Lac-Est and Lac-Rut （P〉0.05）. The test indicated that rutin could improve the level of E2 in lactation rats, increase PRL and GH release, up-regulated the expression level of ER, PR, PRLE and GHR. And finally rutin had weakly estrogen-like effect.关键词
芦丁/大鼠/激素/受体Key words
rutin/rat/hormone/receptor分类
生物科学引用本文复制引用
郭旭东,刁其玉,王月影,屠焰,闫贵龙,汪新建,李军涛,邓东晓..芦丁对哺乳大鼠乳腺发育及相关激素水平与受体表达量关系的影响[J].中国农业大学学报,2011,16(5):88-95,8.基金项目
国家“十·五”科技支撑计划项目（2006BAD1280404) （）
