中国农业大学学报2012，Vol.17Issue(1)：20-25,6.
小麦钙联蛋白基因TaCNX600.的克隆与表达分析
Molecular cloning of wheat calnexin gene TaCNX60.0 and analyzing of its expression
摘要
Abstract
Calnexin is one of the molecular chaperone molecules with highly conserved structures and biological functions.It plays crucial roles in the regulation processes of biological metabolism and Ca2＋ signal transduction.In this study,we screened a cDNA library of a near-isogenic line of wheat stripe rust resistance gene Yr5 and cloned a calnexin gene named TaCNX60.0.Sequence analysis showed that the cDNA fragment contained a 1 921-bp open reading frame,and was predicted to encode a protein with 535 amino acids.Semi-quantitative RT-PCR was performed to investigate the gene expression profiles when wheat plants were treated with different stress conditions and after inoculated with wheat pathogens.It was found that TaCNX60.0 expression was suppressed by exogenous phytohormone ABA,cold and drought treatments.However,it was induced after infection by a strain of Puccinian striiformis CYR32 and mixed races of Erysiphe graminis f.sp.triticis.These results indicated that TaCNX60.0 may involve in wheat defense and stress responses.关键词
小麦/钙联蛋白/分子伴侣/条锈菌/逆境胁迫Key words
wheat/calnexin/molecular chaperone/Puccinia striiformis/abiotic stress分类
农业科技引用本文复制引用
邢晶莉,王凤涛,安艳秋,关尔鑫,蔺瑞明,冯晶,郭玉华,徐世昌..小麦钙联蛋白基因TaCNX600.的克隆与表达分析[J].中国农业大学学报,2012,17(1):20-25,6.基金项目
转基因生物新品种培育重大专项(2009ZX08009-053B) （2009ZX08009-053B）
国家自然基金国际合作项目(30821140351) （30821140351）
国家"973"计划项目(2011CB100403) （2011CB100403）
公益性行业专项(200903035) （200903035）
