中国农业大学学报2012,Vol.17Issue(1):72-79,8.
‘峰后’葡萄果实发育与赤霉素(GA_3)处理以及VvSEP3基因表达的关系
Berry development in relation to Gibberellin(GA_3) treatment and VvSEP3expression of 'Fenghou' grapevine
摘要
Abstract
This paper is concerned with The effects of gibberellins(GA3) on berry growth and the possible role of VvSEP3 gene in early berry development of 'Fenghou' grapevine(Vitis vinifera L.).The inflorescences before flowering were treated with 0.1mg/L GA3.The VvSEP3 gene expression pattern during early berry development was studied by in situ hybridization.Our results showed that GA3 treatment increased the fruit setting rate and the fruit form index.The longitudinal diameter of GA3 treatment berry was larger than the control from 14 days after full bloom(DAFB) to 56 DAFB.Anatomical structure of the berry showed that the cell longitudinal diameter of the mesocarp was significantly larger than control berry from 7 DAFB,indicating that berry elongation due to GA3 treatment might be the stimulation of cell vertical growth.The VvSEP3 gene was expressed in the stigma,style,diaphragm and the nucellar tissue of ovules during flowering,in the ovary wall after flowering,and in vascular tissues and endosperm tissues during young embryo development.These results suggested that the VvSEP3 gene might play a role in the ovary wall cell division and enlargement process,and also involved in the process of seed formation and development.关键词
葡萄/果实发育/赤霉素处理/VvSEP3基因Key words
grape(Vitis×Labruscana Bailey)/fruit development/GA treatment/VvSEP3 gene分类
农业科技引用本文复制引用
杨玉艳,王建楼,鲁井云,秦旭冬,李天忠,胡建芳..‘峰后’葡萄果实发育与赤霉素(GA_3)处理以及VvSEP3基因表达的关系[J].中国农业大学学报,2012,17(1):72-79,8.基金项目
国家自然科学基金资助项目(30971981) (30971981)
新疆维吾尔自治区科技计划项目(200931101-2) (200931101-2)