中国农业大学学报2012，Vol.17Issue(1)：80-87,8.
葡萄SCARECROW基因家族的分析与表达
Putative SCARECROW gene family of Vitis vinifera L. and their differential expression under GA treatment
摘要
Abstract
Gibberellins（GA） are important plant hormone which promotes the enlargement of seedless grape berries.GA signal transduction,especially downstream elements,in the process of grape berry enlargement is still poorly known.Our previous proteomic results demonstrated that a SCARECROW（SCR） protein was up-regulated in GA3 treated seedless grape berries.As a mining step,11 sequences were identified from the grape genome as putative SCR gene,based on their coding protein＇s similarity to SCR identified in other plants.Specific primers were designed after BLAST analysis.Bunches of Centennial seedless were treated with 30mg/L GA3 on 12 days after flowering（D）.The fruit were collected at the first rapid（13,15 and 19 d）,slow（45 d） and the second rapid growth stage（61 d）.Semi-quantitative RT-PCR showed that these 11 genes were of different transcription levels in mature leaves,young leaves,inflorescences,root tips,shoot tips,dormant buds,embryonic callus（EC） and non-embryonic callus（NEC）.And these putative SCR genes,were up-regulated on 13,19,45,61 d by GA3 treatment.Our results lay the ground for future work on full sequence clone and functional study of grape SCR genes.关键词
葡萄/SCARECROW/GA3处理/半定量/基因家族Key words
grape/SCARECROW/GA3/semi-quantitative RT-PCR/gene family分类
农业科技引用本文复制引用
陈丽华,葛晖,柴丽娟,陈尚武,马会勤..葡萄SCARECROW基因家族的分析与表达[J].中国农业大学学报,2012,17(1):80-87,8.基金项目
中国农业大学基本科研业务费资助项目(2009JS51) （2009JS51）
