中国农业大学学报2012，Vol.17Issue(1)：100-104,5.
菊花BRC1b基因的分离与亚细胞定位
Isolation and expression analysis of BRANCHED1 in Dendranthema grandiflora ＇Jinba＇
摘要
Abstract
A BRANCHED1-like gene named DgBRC1b was cloned by RT-PCR and rapid amplification of cDNA ends（RACE） using Dendranthema grandiflora ＇Jinba＇.A predicated 1 008 bp open reading frame was deduced in the cloned sequence,which encoded 335 amino acids.By alignment analysis,the deduced amino acid sequence contained two conserved regions which was identified as diagnostic of true TCP.The gene was proposed to belong to TB1 subfamily by phylogenetic analysis.Subcellular localization revealed that DgBRC1b was localized preferentially distributed to nucleus.关键词
菊花/侧枝发育/TCP家族/亚细胞定位/RT-PCRKey words
chrysanthemum/branching/TCP/subcellular localization/RT-PCR分类
农业科技引用本文复制引用
周晓阳,陈晓丽,毕玲,马男,赵梁军..菊花BRC1b基因的分离与亚细胞定位[J].中国农业大学学报,2012,17(1):100-104,5.基金项目
农业部‘948’项目(2011-G17) （2011-G17）
公益性行业(农业)科研专项经费资助(200903020) （农业）
