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碳氮源对灵芝液体发酵胞外糖肽产量的影响

袁保京 张日俊

中国农业大学学报2012,Vol.17Issue(1):119-124,6.
中国农业大学学报2012,Vol.17Issue(1):119-124,6.

碳氮源对灵芝液体发酵胞外糖肽产量的影响

Effect of carbon and nitrogen source on exopolysaccharide peptides production of Ganoderma lucidum in submerged culture

袁保京 1张日俊1

作者信息

  • 1. 中国农业大学动物科学技术学院/动物营养学国家重点实验室/饲料生物技术实验室,北京100193
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摘要

Abstract

The purpose of this study is to discover high efficient and economical raw materials for commercial production of G.lucidum exopolysaccharide peptides(EPSP) in submerged culture.Twelve different souces(six carbon and six nitrogen sources) were used individually in 250 mL shake flask.The optimal carbon source was cornmeal for both EPSP production((2.664±0.801) g/L) and cell growth((4.559±0.150) g/L),the best nitrogen source was urea for EPSP production((0.636±0.040) g/L),and the most favorable nitrogen source was soybean powder for cell growth((2.222±0.256) g/L).Subsequently,the most advantageous combination in EPSP yield was cornmeal+soybean powder,which resulted in up to(4.366±0.434) g/L.The result of this study would be beneficial to industrial production of EPSP from G.lucidum.

关键词

灵芝/胞外糖肽/碳源/氮源/液体深层发酵

Key words

Ganoderma lucidum/exopolysaccharide peptides/carbon source/nitrogen source/submerged culture

分类

农业科技

引用本文复制引用

袁保京,张日俊..碳氮源对灵芝液体发酵胞外糖肽产量的影响[J].中国农业大学学报,2012,17(1):119-124,6.

基金项目

动物营养学国家重点实验室资助项目(2004DA1251840810) (2004DA1251840810)

中国农业大学学报

OA北大核心CSCDCSTPCD

1007-4333

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