中国农业科技导报2011，Vol.13Issue(6)：12-19,8.DOI:10.3969/j.issn.1008-0864.2011.06.03
新型筛选标记磷酸甘露糖异构酶基因在转基因植物中的应用
Applications of Phosphomannose Isomerase-based Selection System in Transgenic Plants
摘要
Abstract
Selective marker gene encoding antibiotic and herbicide, widely used in plant transformation, have potential risk for environment and human health. Therefore, non-resistance marker genes with bio-safety become a research hotspot in the area of transgenic plants. This paper presented a novel selective system for plant transformation , which utilizes the pmi gene encoding phosphomannose-isomerase as selective marker gene, and summarized the progresses of PMI on its function principle, bio-safety evaluation, application and detection in plant transformation. The paper also analyzed the existing problems of PMI as selective marker gene, and prospected the development trends of PMI, so as to establish a solid foundation for its extensive utilization in transgenic plants.关键词
磷酸甘露糖异构酶(PMI)/转基因植物/选择标记/生物安全性/遗传转化Key words
phosphomannose isomerase(PMI)/transgenic plant/selective marker/bio-safety/genetic transformation分类
农业科技引用本文复制引用
郭倩倩,孙熙森,唐益雄,吴燕民..新型筛选标记磷酸甘露糖异构酶基因在转基因植物中的应用[J].中国农业科技导报,2011,13(6):12-19,8.基金项目
转基因生物新品种培育重大专项(2009ZX08005-004B)资助. （2009ZX08005-004B）
