中国农业科技导报2011，Vol.13Issue(6)：41-45,5.DOI:10.3969/j.issn.1008-0864.2011.06.07
植物基因打靶中正负筛选系统的研究
Studies on Positive-negative Selection System in Plant Gene Targeting
摘要
Abstract
Gene targeting is one of the important tools in reversed genetics and a powerful tool in functional characterization of plant genes. Positive-negative selection system is used to identify correct transformants upon gene targeting. Using selection systems can reduce work load and greatly improve gene targeting efficiency. In recent years, with continuous studies and improvement, the use of positive-negative selection in plant gene targeting has become normal. This paper expounds the principle of the method and summarized its research progress, which will be of significant value for gene targeting study in the future.关键词
基因打靶/正负筛选/codA/DT-AKey words
gene targeting/positive-negative selection/codA/DT-A分类
生物科学引用本文复制引用
李珊珊,安书红,刘昱辉..植物基因打靶中正负筛选系统的研究[J].中国农业科技导报,2011,13(6):41-45,5.基金项目
国家863计划项目(2007AA100503)资助. （2007AA100503）
