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土壤水分胁迫对不同花生种子蛋白质组成类型的影响

宋文武 丁红 陈殿绪 孙奎香 张玉凤 张智猛

中国农业科技导报2011,Vol.13Issue(6):118-125,8.
中国农业科技导报2011,Vol.13Issue(6):118-125,8.DOI:10.3969/j.issn.1008-0864.2011.06.19

土壤水分胁迫对不同花生种子蛋白质组成类型的影响

Effect of Soil Water Stress on Different Composition Types of Peanut Seed Protein

宋文武 1丁红 1陈殿绪 1孙奎香 1张玉凤 1张智猛1

作者信息

  • 1. 山东省花生研究所,山东青岛266100
  • 折叠

摘要

Abstract

To understand the drought resistance mechanism of peanut and screen peanut genotypes with drought resistance and high water use efficiency, the protein composition, protein types and corelation of different peanut varieties were studied under moderate soil water stress condition and by artificial control. The results showed that albumin was preponderant in peanut seed protein, accounting for over 92% , with the highest 95% of the total peanut seed protein. The globulin content was relatively low, accounting for 4% of the total peanut protein. The glutenin content was the lowest, only taking 3. 43% , and the prolamin was trace amount. Among these 4 components, the globulin contents were the most different among varieties. The average concentration of peanut kernels protein component was slightly increased under drought stress, for which albumin content played very important role. The SDS-PAGE indicated that the protein compositions of peanut seeds were determined by genetic characteristics. Soil moisture did not affect the electrophoresis pattern of protein composition of same species (lines) , but influence the depth of their strips. The profiles of albumin and globulin bands were darker under soil moisture stress indicating an increase of responsive protein components. The albumin protein bands under treatment and the globulin bands of No. 29 variety were increased obviously, a intensive response to drought.

关键词

花生/水分胁迫/蛋白质/组成类型/影响

Key words

peanut/water stress/protein/composition type/effect

分类

农业科技

引用本文复制引用

宋文武,丁红,陈殿绪,孙奎香,张玉凤,张智猛..土壤水分胁迫对不同花生种子蛋白质组成类型的影响[J].中国农业科技导报,2011,13(6):118-125,8.

基金项目

国家科技支撑计划(2006BAD21B04-2 ()

2009BADA8B03) ()

青岛市科技支撑项目(08-2-1-22-nsh) (08-2-1-22-nsh)

现代农业产业技术体系建设专项资金(CARS-14)资助. (CARS-14)

中国农业科技导报

OA北大核心CSCDCSTPCD

1008-0864

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