中国农业科学2011，Vol.44Issue(22)：4593-4599,7.DOI:10.3864/j.issn.0578-1752.2011.22.005
小麦秆锈菌特异性SSR分子标记的开发
Development of a SSR Molecular Marker for Puccinia graminis f.sp.tritici
摘要
Abstract
[Objective] The objective of this study is to build a simple, quick and accurate molecular technique to detect Puccinia graminis f. Sp. Tritici and forecast this destructive disease. [Method] A microsatellite-enriched genomic library of Pgt was constructed by using the method of FIASCO. Based on the sequence of Pgt microsatellite, pairs of specific SSR primers were developed and screened. [Result] The primer Pgtfssrl (fir) generated a polymorphic pattern displaying a 395 bp DNA fragment specific for Pgt, whereas no DNA fragment was obtained in other 24 non-target wheat fungal pathogens. The existence of specific DNA fragment was detected in the infected wheat tissues at 30 h post-inoculation, and the sensitivity of this molecular marker was Pgt DNA template of 1 ng-uL'1. [Conclusion] A specific SSR marker for the detection of wheat stem rust has been successfully developed, which could be used for the early diagnosis and forecast of wheat stem rust.关键词
小麦秆锈菌/微卫星标记/FIASCO/分子检测Key words
Puccinia graminis f. Sp. Tritici/ microsatellite marker/ FIASCO/ molecular detection引用本文复制引用
王曦,刘太国,向文胜,陈万权..小麦秆锈菌特异性SSR分子标记的开发[J].中国农业科学,2011,44(22):4593-4599,7.基金项目
国家重点基础研究发展计划"973"项目(2011CB100403,2010CB951503)、国家公益性行业(农业)科研专项(200903035)、国家小麦产业技术体系(CARS-03-04B) （2011CB100403,2010CB951503）
