中国农业科学2011，Vol.44Issue(22)：4610-4617,8.DOI:10.3864/j.issn.0578-1752.2011.22.007
长期施肥对红壤性水稻土细菌群落结构和数量的影响
Response of Abundance and Composition of the Bacterial Community to Long-term Fertilization in Paddy Soils
摘要
Abstract
[ Objective] The impacts of long-term fertilization on soil microbial community structure and abundance were studied to clarify their relationship and provide a theoretical basis for the soil fertility evaluation and management of rice cultivation. [Method! The soil samples were collected from long-term fertilization experimental plots receiving no fertilization, chemical fertilization, chemical fertilizer + crop residue incorporation. The terminal restriction fragment length polymorphism (T-RFLP) and real-time quantitative PCR analysis were combined to characterize the bacterial community structure and abundance. [Result] Proteobacteria (150 bp; relative abundance 33%-37%) and actinomycetes (67 bp; relative abundance 20%-25%) dominated the bacterial community in paddy soils based on T-RFLP analysis. There was a pronounced difference in the bacterial community composition and diversity responding to the long-term fertilization regimes. CCA results indicated that soil organic carbon (SOC) and Ph had the greatest influence on the bacterial community composition. The diversity analysis of Shannon and Evenness Index showed that compared with the unfertilized control, all fertilization treatments significantly increased the bacterial diversity and the harvested residue incorporation also shared the highest. The abundance of bacterial 16S Rdna ranging from 4.34xlO10-10.94xl010 copies-g"'soil was determined by real-time PCR, and the amount of 16S Rdna copy number in the fertilization incorporations increased about 50% to 100% of which in unfertilized treatments [Conclusion] Long-term chemical fertilizer and crop residueincorporation improved soil quality and fertility, and consequently resulted in the shift of the bacterial microecology and promoted the bacterial diversity and abundance.关键词
稻田/施肥/细菌群落结构/多样性/T-RFLP/real-time PCRKey words
paddy soil/ fertilization/ bacterial community structure/ diversity/ T-RFLP/ real-time PCR引用本文复制引用
袁红朝,秦红灵,刘守龙,童成立,魏文学,吴金水..长期施肥对红壤性水稻土细菌群落结构和数量的影响[J].中国农业科学,2011,44(22):4610-4617,8.基金项目
国家自然科学基金项目(40901124,40701083,40801098),中科院知识创新工程青年人才领域前沿项目(ISACX-LYQY-QN-0702) （40901124,40701083,40801098）
