中国农业科学2011，Vol.44Issue(22)：4645-4652,8.DOI:10.3864/j.issn.0578-1752.2011.22.011
蔷薇属植物类根体和类原球茎发生发育的影响因子
Factors Influencing the Occurrence and Development of Rhizoid and Protocorm-Like Body in Rosa spp.
摘要
Abstract
[Objective] Leaf explants of multiple species of Rosa spp. Were used for investigation of the factors influencing the occurrence and development of rhizoid and protocorm-like body in this paper, which can lay a foundation for revealing the occurrence mechanism of PLB and molecular breeding. [ Method ] The growth of rhizoid and protocorm-like body were observed and statistics were made by changing the medium components, different growth regulators and their concentrations, culture conditions and the integrity of the rhizoid on the Murashige and Skoog (MS) medium. [Result] The results showed that the occurrence and size of the rhizoid was different depending on the type of auxin, but not cytokinin. Rhizoid could be induced from leaf explants of Rosa spp. On the Murashige and Skoog (MS) medium containing any type of the three auxins. On the other hand, rhizoid could be induced from the leaf explants only in dark, however, this process was significantly suppressed under light condition. On the contrary, lighting promoted the matured rhizoid turning green and developed into protocorm-like body(PLB), but this process did not happen under dark condition and light-dark condition without TDZ. Cutting part of the matured rhizoid from the top, the rhizoid could also develop into PLBs, whereas, the rhizoid cut from the callus surface, couldn't grow into PLBs, but rather turnbrown and died, whether they were placed horizontally or vertically. This indicated that the tip of the rhizoid was not essential for the PLBs formation, but the bottom of rhizoid was vitally important for TDZ absorption, loss of the callus on the rhizoid bottom affected the absorption and transportation of TDZ, and further, the development of the protocorm-like body was suppressed. [Conclusion] This method is of universality for the rhizoid induced by auxin. Lighting is a inhibitor for the occurrence of the rhizoid but a promoting factor for the information of the PLB, lighting and TDZ has a synergistic action on the process of rhizoid developing into protocorm-like body. The tip of rhizoid is not the essential for the PLBs formation, but the bottom of rhizoid is very important for TDZ absorption.关键词
蔷薇属植物/组织培养/类根体/类原球茎/发生与发育Key words
Rosa spp./ tissue culture/ rhizoid/ protocorm-like body(PLB)/ occurrence and development引用本文复制引用
张建甫,毕玲,陈晓丽,郭艳超,赵梁军..蔷薇属植物类根体和类原球茎发生发育的影响因子[J].中国农业科学,2011,44(22):4645-4652,8.基金项目
国家自然科学基金项目(30871733) （30871733）
