中国农业科学2011，Vol.44Issue(23)：4926-4932,7.DOI:10.3864/j.issn.0578-1752.2011.23.020
鼠李糖乳酸杆菌对Caco-2细胞抗氧化功能的影响
Antioxidative Function of Lacbacillus rhamnosus to Caco-2 Cells
摘要
Abstract
[Objective] This experiment was conducted to study the antioxidative function of Lactobacillus rhamnosus to Caco-2 cells under oxidative stress. [Method] Caco-2 cells were randomly divided into 4 groups, control group and oxidative stress group (100 umol·L-1 H2O2), treatment groups I and II were added with 1 Ml Lactobacillus rhamnosus (about 108 CFU·Ml-1) and tertiary butylhydroquinone (TBHQ) (2.75 μg·Ml-1 ) under oxidative stress, respectively. Antioxidative activities in culture supernatants and lysates of Caco-2 cells at 12 h and 48 h were measured. [Result] Compared to oxidative stress group, the total antioxidation capacity (T-AOC), the glutathione peroxidase (GSH-Px) (P<0.01), catalase (CAT) (P<0.01) activities in the cultured supematants, and the superoxide dismutase (SOD) activity (P<0.01), and the glutathione (GSH) content (P<0.05) in the cells lysates at 12 h in treatment group I increased, and the increases of the antisuperoxide anion radical (O2) (P<0.01), GSH-Px (P< 0.01), CAT (.P<0.01), SOD (P<0.0l) activities in the cells culture supernatants and the peroxidase (POD) activity (P<0.01) and the decreases of malondialdehyde (MDA) content (P<0.01) in the cells lysates at 48 h were also observed. In treatment group II, the T-AOC (P<0.01), CAT activity (P<0.01) in the cells culture supematants and the glutathione (GSH) content (JP<0.01) in the cellslysates at 12 h increased significantly, but these values and the MDA content at 48 h decreased significantly (P<0.01). The anti-superoxide anion radical (O2), SOD, GSH-Px, POD activities in the cells culture supernatants and the POD activity in the cells lysates at 48 h increased (P<0.05). [Conclusion] These results indicated that adding Lactobacillus rhamnosus could increase the antioxidative function of Caco-2 cells under oxidative stress under in vitro conditions.关键词
Caco-2细胞/鼠李糖乳酸杆菌:TBHQ/抗氧化活性/氧化应激Key words
Caco-2 cells/ Lactobacillus rhamnosus/ TBHQ/ antioxidative activities/ oxidative stress引用本文复制引用
崔志文,黄琴,黄怡,吴红照,文静,李卫芬..鼠李糖乳酸杆菌对Caco-2细胞抗氧化功能的影响[J].中国农业科学,2011,44(23):4926-4932,7.基金项目
国家重点基础研究发展计划(2009CB118705)、浙江省重大科技专项(2006C12086) （2009CB118705）
