中国农业科学2011，Vol.44Issue(23)：4952-4961,10.DOI:10.3864/j.issn.0578-1752.2011.23.024
超表达番茄GDP-D-甘露糖焦磷酸化酶基因马铃薯对温度胁迫的响应
Effect of Tomato GMPase Overexpression on Tolerance of Potato Plants to Temperature Stress
摘要
Abstract
[Objective] The aim of the study was to clarify the function of the GDP-D-mannose pyrophosphorylase (GMPase) in the L-galactose pathway of ascorbic acid (AsA) synthesis in plants, and to make sure the relation of GMPase overexpression with ascorbic acid content and temperature-stress tolerance ability. [Method] Two transgenic potato strains of overexpressing tomato GMPase were obtained by agrobacterium-mediated transformation. The transgenic plants and wild-type plants were treated in temperature-stress conditions (10/5℃ as low-temperature stress, 35/30°C as high-temperature stress) for 3 days then in the recover-condition (25/20℃) for 3 days. Real time RT-PCR was used to analyze the expression of GMPase gene. And ascorbic acid content and relative index to temperature stress were also determined. [Result] Compared with wild-type plants, with the overexpression of GMPase gene in transgenic plants, the GMPase activity and contents of AsA and dehydroascorbate (DHA) in normal and temperature-stress conditions were increased as well as the activities of dehydroascorbate reductase (DHAR), monodehydroascorbate reductase (MDHAR) and ascorbate peroxidase (APX). Meanwhile, the malondialdehyde (MDA) and H2O2 contents and permeability were decreased in the transgenic plants. [Conclusion] The overexpression of GMPase may increase potato tolerance to temperature stress by increasing AsA level and lowering reactive oxygen species (ROS).关键词
马铃薯/抗坏血酸/GDP-甘露糖焦磷酸化酶/转基因/温度胁迫Key words
potato/ ascorbic acid/ GDP-D-mannose pyrophosphorylase/ transgenic/ temperature stress引用本文复制引用
李超汉,李青竹,史庆华,白龙强,郭晓青,李霞,于贤昌..超表达番茄GDP-D-甘露糖焦磷酸化酶基因马铃薯对温度胁迫的响应[J].中国农业科学,2011,44(23):4952-4961,10.基金项目
国家自然科学基金项目(30771473)、国家科技支撑计划项目(2011BAD12B01)、农业部园艺作物遗传改良重点开放实验室项目 （30771473）
