中国农业科学2011，Vol.44Issue(24)：5067-5072,6.DOI:10.3864/j.issn.0578-1752.2011.24.012
猪POU1F1基因启动子区多态分析及其与生长性状的关联
Polymorphism Analysis of POU1F1 Promoter Region and It's Association with Growth Traits
摘要
Abstract
[Objective ] The aim of current study is to obtain the polymorphisms of POU1F1 promoter region, investigate the distribution of these polymorphisms among different pig breeds and detect it's association with growth traits. [Method] PCR cloning, DNA sequencing and PCR-RFLP techniques were applied to detect the polymorphisms of POU 1F1 promoter region, and the general linear model was used to analyze it's association with growth traits. [Result] Five polymorphism sites were identified in the 1.5 kb POU1F1 promoter region, and the frequency of A allele in the 5 bp insertion or deletion mutation was the highest in introduced pig breeds, moderate in bred breeds, and lowest in Chinese native breeds. The general linear model analysis indicated that this polymorphism site was significantly associated with growth traits. LSD showed that the pigs with AA genotype had higher body highness, body length, circumference and body weight at 6-month-old of age compared with that of AB and BB pigs, but the difference of body highness, circumference and body weight between AB and BB pigs was not significant, while the body length of AB pigs was significantly higher than that of BB pigs. [Conclusion] The A allele in 5bp insertion or deletion mutation site is favorable, and it could be useful in molecular marker assisted selection for growth traits.关键词
猪/POU1F1基因/多态/生长性状Key words
pig/POU1F1 gene/polymorphism/growth traits引用本文复制引用
宋成义,赵芹,高波,王宵燕,吴晗,周辉云,经荣斌,毛九德..猪POU1F1基因启动子区多态分析及其与生长性状的关联[J].中国农业科学,2011,44(24):5067-5072,6.基金项目
江苏省自然科学基金(BK2010317)、江苏省科技支撑计划(农业)(BE2009330-3)、江苏高校优势学科建设工程资助项目(PAPD) （BK2010317）
