中国普通外科杂志2011，Vol.20Issue(12)：1342-1346,5.
骨髓间充质干细胞联合肝细胞生长因子基因治疗兔肢体缺血
Combination treatment with bone mesenchymal stem cells and HGF gene therapy for limb ischemia of rabbit
摘要
Abstract
Objective To investigate the therapeutic effect of the combination treatment of bone mesenchymal stem cells ( BMSC ) and hepatocyte growth factor ( HGF) gene therapy on limb ischemia of rabbits. Methods Ischemic model of hind limb was induced in the right hind limb of 32 New Zealand rabbits by ligation of the deep femoral artery and complete excision of the superficial femoral artery. After the model was established, the rabbits were equally randomized into empty plasmid treatment group ( control group) , BMSC treatment group ( BMSC group ) , HGF gene therapy group ( HGF group ) and BMSC treatment plus HGF gene therapy group ( BMSC + HGF group ) . All treatment regimens were administered by direct intramuscular injection into the operated limb. The collateral vessel count was performed using angiography 28 days after treatment, and the expression of CD 31 and HGF in the muscle tissues surrounding the injection sites was detected by immunohistochemical staining and Western blot analysis respectively, 30 days after treatment. Results The collateral vessel count showed no statistical difference in BMSC group or HGF group compared with control group, but it increased significantly in BMSC + HGF group compared with other 3 groups(P<0.05 or P < 0. 01 ) . The immunohistochemical staining showed that the CD31 expression level of all the treated groups was significantly higher than that of the control group ( P < 0. 05 or P < 0. 01 ) , and CD 31 expression had no significant difference between BMSC group and HCF group, but both of which were significantly lower than that of BMSC + HGF group ( both P < 0. 05 ). The Western blot demonstrated that the HGF expression level of all the treated groups was significantly higher than that of the control group (P<0.05 or P <0. 01 ) , and it was increased in the order of BMSC, HGF and BMSC + HGF group that all had statistical differences (all P < 0. 05 ). Conclusions The combination treatment with BMSC and HGF gene therapy has synergistic effect that can effectively promote collateral vessel development, and thereby improve the perfusion of the ischemic limb of rabbit.关键词
骨髓间充质干细胞/肝细胞生长因子/肢体缺血/血管生成Key words
Bone Mesenchymal Stem Cells/Hepatocyte Growth Factor/Limb Ischemia/Angiogenesis分类
医药卫生引用本文复制引用
周华,王默,金星,张十一,高斌斌,王茂华..骨髓间充质干细胞联合肝细胞生长因子基因治疗兔肢体缺血[J].中国普通外科杂志,2011,20(12):1342-1346,5.基金项目
山东省优秀博士基金资助项目(2006bs03025 （）
2008bs03011) （）
