中国普通外科杂志2012，Vol.21Issue(1)：48-52,5.
Nodal靶向RNA干扰对人肝癌细胞的影响
Effect of nodal targeting RNA interference on human hepatocellular carcinoma cells
摘要
Abstract
Objective: To observe the inhibitory effect of RNA interference (RNAi) on the expression of nodal gene in human hepatocellular carcinoma (HCC) cells, and explore the feasibility of nodal gene targeting in HCC gene therapy.Methods: SMMC-7721 cells were transfected with the plasmid vector containing a short hairpin RNA (shRNA) sequence targeting the nodal gene. After assessing the transfection efficiency, the nodal gene and protein expression was detected by real-time quantitive PCR and Western blot,respectively. Furthermore, the effect of nodal gene interference on the proliferation of SMMC-7721 cells in vitro was studied. Results: The plasmid containing shRNA had excellent transfection efficiency in SMMC-7721 cells. After transfection, the expression of nodal Mrna and protein of SMMC-7721 cells was significantly inhibited, and their proliferation in vitro was also significantly suppressed.Conclusion: The nodal gene and protein expression as well as cell proliferation of HCC cells can be effectively inhibited by RNAi technique, so nodal gene may be a potential therapeutic target for HCC.关键词
癌/肝细胞/RNA/干扰/基因/NodalKey words
Carcinoma, Hepatocellular/RNA,Interference/Gene, Nodal分类
医药卫生引用本文复制引用
陈启斌,荚卫东,许戈良,李建生,马金良,刘文斌,王修军..Nodal靶向RNA干扰对人肝癌细胞的影响[J].中国普通外科杂志,2012,21(1):48-52,5.基金项目
国家自然科学基金资助项目(30972892) （30972892）
安徽省"115"产业创新团队-肝细胞癌转移复发研究团队资助项目(2008-01) （2008-01）
安徽省2011年度科技计划项目(1106c0805028). （1106c0805028）
