中国普通外科杂志2012，Vol.21Issue(1)：67-70,4.
肝硬化组织中p16INK4a和Rb的基因甲基化状态和蛋白表达
Gene methylation status and protein expression of P 16INK4a and Rb in cirrhosis liver tissue
摘要
Abstract
Objective: To investigate the methylation status at the promoter region of the p16INK4a and retinoblastoma (Rb) gene in cirrhosis liver tissue and its relations with the protein expression of the two genes.Methods: The DNA methylation status and protein expression of p 16INK4a and Rb in the liver tissues from 65 patients with cirrhosis and 20 healthy subjects were detected by PCR and immunohistochemical staining,respectively.Results: No abnormal methylation of p16INK4a and Rb gene was detected in any of the 20 cases of normal liver tissue, while the methylation ratios of the p 16INK4a and Rb gene in the cirrhosis liver tissue were 40% (26/65) and 36.9% (24/65), respectively. The integrated optical densities (IOD) of the p16INK4a and Rb protein expression in the normal liver tissue were 225.7±27.4 and 254.7±34.8, while those in the cirrhosis liver tissue were 32.4±7.5 and 45.2±6.4, respectively, and both differences had statistical significance (both P<0.05). Conclusion: Cirrhosis liver tissue has abnormal methylation of pl6INK4a and Rb gene and decreased expression of pl6INIMa and Rb protein, and the resulting loss of cell cycle control may be involved in the occurrence and development of liver cirrhosis.关键词
肝硬化/DNA甲基化/p16INK4a/基因,视网膜母细胞瘤Key words
Liver Cirrhosis/DNA Methylation/p16INK4a/Genes, Retinoblastoma分类
医药卫生引用本文复制引用
王海久,邓勇,王聪,侯立朝,阳丹才让,任利,樊海宁..肝硬化组织中p16INK4a和Rb的基因甲基化状态和蛋白表达[J].中国普通外科杂志,2012,21(1):67-70,4.基金项目
国家自然科学基金资助项目(81060133). （81060133）
