中国普通外科杂志2012，Vol.21Issue(1)：85-89,5.
腑安汤对小鼠术后肠麻痹的影响及机制
The influence of Fuan decoction on postoperative ileus of rat and its mechanism
曲明阳 1孙传欣 1赵胜男 1王莉芬 1邢光明1
作者信息
- 1. 大连医科大学附属第二医院普通外科,辽宁大连116027
- 折叠
摘要
Abstract
Objective: To investigate the therapeutic effect of Fuan decoction on postoperative ileus (POI) and its related mechanisms.Methods: Ninety mice were randomized into sham operated control group, POI model group and Fuan decoction pretreatment group, and then the rats underwent the conventional procedure of POI establishment or sham operation, respectively. The content of tryptase and histamine of the small intestinal tissue was detected by ELISA method. The number of mesenteric mast cells was counted and their degranulation was observed under light microscope after toluidine blue staining. The myeloperoxidase (MPO) activity of the small intestinal tissue was determined by colorimetric method and the carbon powder moving rate was also measured.Results: The content of tryptase and histamine, and the MPO activity of the POI model group were all increased significantly compared with those of the sham operated control group (all P<0.0l), but the elevations of these parameters were remarkably inhibited in the Fuan decoction pretreatment group and these had statistical differences compared with the POI model group (all P<0.0l). No significant difference in the number of mast cells was found among the groups, but the degranulation of mesenteric mast cells was evident in POI model group and was inhibited in Fuan decoction pretreatment group. The carbon powder moving rate was significantly decreased in POI model group after surgery (P<0.01), which was significantly higher in Fuan decoction pretreatment group than that of the POI model group (P<0.0l).Conclusion: Fuan decoction can reduce the production of intestinal tryptase and histamine after surgery and inhibit the degranulation of mast cells and their inducttion of inflammatory response, and thus it has the effect of preventing and alleviating postoperative Ileus.关键词
肠麻痹,手术后/手术后并发症/预防和控制/腑安汤/肥大细胞/炎症趋化因子类Key words
Postoperative Ileus/Postoperative Complications/prev/Fuan Decoction/Mast Cells/Chemokines分类
医药卫生引用本文复制引用
曲明阳,孙传欣,赵胜男,王莉芬,邢光明..腑安汤对小鼠术后肠麻痹的影响及机制[J].中国普通外科杂志,2012,21(1):85-89,5.
