中国人兽共患病学报2011，Vol.27Issue(12)：1082-1085,4.
维氏气单胞菌吉林分离株外膜蛋白AⅡ基因的克隆及生物信息学分析
Cloning and bioinformatics analysis on outer membrane protein gene A Ⅱ of Aeromonas veronii isolated from Jilin Province
摘要
Abstract
The purpose of this study was to clone outer membrane protein gene A II of Aeromonas veronii isolated from Jilin Province and analyze biological information of coding protein. Based on the sequence of published OMPAⅡ gene, a pair of primers was designed to amplify the OMPA Ⅱ gene by PCR technique, and was cloned into PMD18-T vectors. Then the posi-tive recombinant plasmid was identified by PCR and sequencing. At last, the OMPA Ⅱ gene homology among this strain and others was analyzed by BlastN, and the phylogenetic tree was constructed. Meanwhile, the related biological information of the coding protein was analyzed. The result showed that the OMPAⅡ gene was 1001 bp and the homology with others gene se-quences reported by GenBank was 95%. By bioinformatics analysis, the OMPⅡ protein doesn't contain transmembrane region and its N-terminal contains one signal peptide. The secondary structure mainly contains β-pleated sheet and β-turn, and ten re-gions are likely to contain B cell antigenic determinants. The OMPAⅡ gene of Aeromonas Veronii was successfully cloned, and related biological information of the protein was analyzed.关键词
维氏气单胞菌/外膜蛋白AⅡ/克隆/序列分析/生物信息学Key words
Aeromonas Veronii/ outer membrane protein gene AⅡ / gene cloning/ sequence analysis/ bioinformatics分类
医药卫生引用本文复制引用
单晓枫,吴同垒,孟庆峰,王伟利,钱爱东..维氏气单胞菌吉林分离株外膜蛋白AⅡ基因的克隆及生物信息学分析[J].中国人兽共患病学报,2011,27(12):1082-1085,4.基金项目
国家支撑计划项目(2010BAD04B01)、吉林省科技厅项目(20080218)和吉林农业大学青年启动基金项目联合资助 （2010BAD04B01）
单晓枫和吴同垒为同等贡献 （）
