中国人兽共患病学报2011，Vol.27Issue(12)：1097-1100,4.
结核分枝杆菌链霉素耐药临床分离株rpsL基因突变特征分析
Analysis on the characteristics of rpsL gene mutations in Streptomycin resistance isolates of Mycobacterium tuberculosis
摘要
Abstract
The objective was to understand the characteristics of rpsL gene mutation in Streptomycin resistant clinical i-solates of Mycobacterium tuberculosis (M. tuberculosis) in China, and evaluate the application of rapid detection of rpsL gene mutation by PCR. The 302 M. tuberculosis clinical isolates were detected by drug sensitivity test (DST), and the rpsL was de-tected by PCR-directly sequence (PCR-DS). Of 59 strains were pan-susceptible to 5 drugs including INH, RFP, SM, EMB and PAS, and 61 strains were sensitive to streptomycin (SM) but resistant to the other drugs. The mutation rate of SM sus-ceptible isolates was 5. 00% (6/120). In the 182 SM resistant isolates, the rpsL mutation rate was 70. 33% , and the mutation rate of codon43 Lys→Arg was 52. 20%; there were 4 mutation types in codon 88, and the mutation rate was 20. 33% ; the mu-tation rate of codon 86 Arg→Gln was 0. 55%. The rpsL gene mutation rate of SM resistant strains was significantly higher than that of SM-sensitive strains x2= 125. 04,P<0. 05. In conclusion, the rpsL mutation in M. tuberculosis is highly associated with Streptomycin resistance, and codon 43 Lys→Arg mutation is the main and important type. Meanwhile, the PCR-DS could be applied to rapid detection of rpsL mutation in clinical M. tuberculosis isolates for identifying SM susceptibility.关键词
结核分枝杆菌/链霉素/耐药/基因突变Key words
Mycobacterium tuberculosis/Streptomycin/drug resistance/rpsL gene分类
医药卫生引用本文复制引用
魏淑贞,赵秀芹,刘志广,万康林..结核分枝杆菌链霉素耐药临床分离株rpsL基因突变特征分析[J].中国人兽共患病学报,2011,27(12):1097-1100,4.基金项目
国家"十一五"重大传染病防治科技重大专项"结核病传播模式研究"(结核病分子流行病学研究,2008ZX100/03-010-02)和福建省卫生厅青年科研课题资助计划(2009-1-26)联合资助.魏淑贞和赵秀芹为同等贡献. （结核病分子流行病学研究,2008ZX100/03-010-02）
