中国人兽共患病学报2011，Vol.27Issue(12)：1115-1118,4.
幽门螺杆菌对甲硝唑的耐药性分析
Analysis on metronidazole-resistance in Helicobacter pylori
摘要
Abstract
To investigate the relationship between the related gene mutation and the resistance in Helicobacter pylori (H. pylori) to metronidazole (Mtz), all the H. pylori strains were isolated from the gastric mucosal biopsies of 60 patients, and incubated in microaerophilic culture. Minimal inhibitory concentration (MIC) of H pylri to Mtz was determined by E-test method. The gene fragments of rdxA.frxA and fdxB were amplified by PCR and then sequenced. Finally polymorphism analy-sis was performed in rdxA, frxA and fdxB from those strains resistant to Mtz and standard strains H. pylori 26695. It was found that there were 11 Mtz-resistant H. pylori strains(MIC≥256 μg/mL) isolated from 60 spesiments of gastric musosa. The gene fragments of rdxA, frxA and fdxB were amplified successfully between 11 stains resistant to Mtz and the standard strains H. pylori 26695. Compared with the standard strains H. pylori 26695, these gene mutation reprsented regularfy that there were not only common mutable points, but also some other random mutable points dispersedly. The results imply that H. pylori's resistance to Mtz is relevant to mutation of rdxA, fdxB and frxA.关键词
幽门螺杆菌/甲硝唑/耐药基因Key words
Helicobacter pylori/ metronidazole/ resistance gene分类
医药卫生引用本文复制引用
张姝,莫非,孙朝琴,张然,王莹,李寅,罗昭逊,夏曙华..幽门螺杆菌对甲硝唑的耐药性分析[J].中国人兽共患病学报,2011,27(12):1115-1118,4.基金项目
贵州省科学技术基金(黔科合J字[2008]2158号)资助 （黔科合J字[2008]2158号）
