摘要
Abstract
Our aim was to understand the resistance situation of second-line anti-TB (tuberculosis) drugs in the patients with multiple drug-resistance tuberculosis (MDR-TB), and evaluate the clinical application for the drug susceptibility test of second-line anti-TB (tuberculosis) drugs by BACTEC MGIT960 system. The clinical Mycobacterium tuberculosis CM. tuberculosis) isolates from the patients with MDR-TB identified by PNB/TCH culture and the susceptibility testing on first-line drugs were collected in Fuzhou Pulmonary Hospital, Fujian Province. Drug susceptibility on 4 second-line drugs including ofloxacin (OFLX), levofloxacin (LVFX), amikacin (AMK) and capreomycin (CPM) were tested by means of the BACTEC MGIT960 system and compared improved L-J medium. Results showed that from October 2008 to April 2009, total of 70 MDR-TB patients, in which there were 26 initial cases and 44 retreated cases, were tested for drug resistance to the 4 second-line drugs. The total drug-resistance rate of the four drugs was 61. 4% (43/70). The drug-resistance rate to OFLX, LVFX, AMK and CPMwas48.6%, 40.0%, 17. 1% and 7. 1% , respectively. The drug-resistant rate of the first treated cases and the retreated cases was 57. 7% (15/26) and 63. 6% (28/44) respectively. There were 5 XDR-TB cases accounting for 7. 1 % , including 1 initial case (1. 4%) and 4 retreated cases (5. 7%). The average report time of the drug susceptibility tests of second-line anti-TB drugs by BACTEC MGIT960 system was only 10. 2 d, which was shorter than that of improved L-J medium. Identical proportion of drug susceptibility tests was higher than 90%. It's concluded that drug-resistance rate of the 4 drugs is in high level within MDR-TB patients who were treated in the hospital. It is needed to pay more attention to XDR-TB control and treatment because there is XDR-TB in Fujian Province. Meanwhile, BACTEC MGIT960 system is an accurate method for rapid testing of susceptibilities of M. tuberculosis to second-line drugs.关键词
结核病/耐多药结核病/广泛耐药结核病/药物敏感性试验Key words
tuberculosis/ multi-drug resistant tuberculosis/ extensively drug resistant tuberculosis/ drug sensitivity test分类
医药卫生
