中国人兽共患病学报2012，Vol.28Issue(1)：51-55,5.
银星竹鼠巨噬细胞对马尔尼菲青霉分生孢子的吞噬作用的实验研究
Phagocytosis of Rhizomys pruinosus macrophage to Penicillium marneffei conidia
摘要
Abstract
The objective of this study was to isolate macrophages from Rhizomys pruinosus, and provide methods for preliminary identification of macrophages based on morphological and phagocytic characteristics. Meanwhile, this research also aimed at observing the phagocytosis of different sources of macrophages to Penicillium marneffei conidia and detecting the phagocytic rate, which will provide initial experimental evidence of the correlation among Penicillium marneffei, Penicilliosis marneffei and Rhizomyidae. Different methods were used to isolate macrophages from alveoli, peritoneum and spleen of Rhizomys pruinosus. The cell morphology was observed by Wright-Giemsa staining and transmission electron microscopy. By transmission electron microscopy, the phagocytosis of different sources of macrophages to Penicillium marneffei conidia and the changes of the surface and intracellular structure of macrophages before and after phagocytosis were observed. The phagocytic rate was detected by flow cytometry. Results displayed that different sources of primary macrophages of Rhizomys pruinosus were isolated. The isolated cells had the characteristic of macrophages in morphology which have been confirmed by light microscope and electron microscope. At the same time, the number of cell surface projection increased and became thicker. However, the number and structure of mitochondria, endocytoplasmic reticulum and lysosome did not change after the phagocytosis. The structure of Penicillium marneffei conidia had no change, either. The phagocytic rates of alveolar, peritoneal and spleen macrophages to Penicillium marneffei conidia were 75. 09%±6. 92%, 83. 36% ±0. 87% , and 78. 84% ±3. 73% , respectively, with statistically significant difference among these three groups (P＜0. 05). It's implied that the macrophages I-solated from alveoli, peritoneum and spleen of Rhizomys pruinosus have good phagocytosis to Penicillium marneffei conidia. No lethal effect of the Rhizomys pruinosus macrophages on Penicillium marneffei conidia was observed by transmission electron microscopy.关键词
银星竹鼠/巨噬细胞/马尔尼菲青霉/分生孢子/吞噬Key words
Rhizomys pruinosus/ macrophage/ Penicillium marneffei/ conidia/ phagocytosis分类
医药卫生引用本文复制引用
张勇,胡永轩,李希清,杨亚波,张军民,鲁莎,席丽艳..银星竹鼠巨噬细胞对马尔尼菲青霉分生孢子的吞噬作用的实验研究[J].中国人兽共患病学报,2012,28(1):51-55,5.基金项目
NSFC-广东联合基金重点项目(U0932003) （U0932003）
