中国人兽共患病学报2012，Vol.28Issue(2)：111-115,5.
汉坦病毒M、S基因分型及种系进化分析
Detection and genotyping of hantavirus M and S gene and its phylogenetic analysis
摘要
Abstract
To investigate the genotype and characteristic of hantavirus M and S hemorrhagic fever with renal syndrome (HFRS) , lung tissues of rats were screened by immunofluorescent antibody assay (IFA) , and the total RNA was extracted from the serum of HFRS patients and IFA positive lung tissues. RT PCR was applied to amplify the M and S gene of hantavir us after specific primers were designed. PCR positive product was recovered and purified before cloning to the pMD 18 T vector and the sequencing was carried by ABI3730 gene sequencer. The obtained sequencings were compared to the HTNV and SEOV sequences of hantavirus for nucleotide homology analysis and applied MEGA5. 0. 4 software to construct the phylogenetic tree. The yellow green needle like particles were scattered in the positive lung tissues under fluorescence microscope. The PCR prod ucts of M and S gene was about 490bp and 593bp with general primers and the further amplification with HTNV typing primers to the M and S gene was respectively 242bp and 276bp, while the amplified results of M and S gene were negative with the primers of SEOV. The sequences of HunanOl, HunanO2 and HunanO3 had a high nucleotide homology to the 84FLi and SN7 strains,which had a lower homology to the Gou3 strain. HunanOl, HunanO2 and HunanO3 were belonged to H5 subtype of HTNV. In conclusion, the technology of genotyping is appropriate for hantavirus diagnosis, and the HTNV patients and ani mal hosts are exist in Hunan Province.关键词
肾综合征出血热/汉坦病毒/M基因/S基因/分型/种系进化Key words
HFRS/hantavirus/ M gene/ S gene/genotyping/ phylogenetic分类
医药卫生引用本文复制引用
蔡亮,张红,高立冬,刘运芝,胡世雄,刘富强,曾舸,刘佳惠,李俊华..汉坦病毒M、S基因分型及种系进化分析[J].中国人兽共患病学报,2012,28(2):111-115,5.基金项目
湖南省卫生厅科研基金(No.A2007008)和湖南省科技厅科研基金(No.2010TD2027)联合资助 （No.A2007008）
