中国人兽共患病学报2012，Vol.28Issue(2)：120-123,147,5.
猪轮状病毒OSU株的培养特性与致病性研究
Culture characteristics and pathogenicity of porcine rotavirus OSU strain
摘要
Abstract
Porcine rotavirus was cultured successfully in MA 104 cells. The virus was treated for lh by trypsin on final concentration of 20μg/niL, and then inoculated in maintain medium with 4μg/mL trypsin. The cytopathic effects such as kyto plasm connecting, botryoidalis accretion and net balloon were clearly observed. Double capsid virus was detected by electronmi croscope. Healthy 3 day old colostrums deprived piglets were inoculated orally with 1X10(6. 8) TCID50 of porcine rotavirus. Piglets developed watery diarrhea l0h post infection, and died in 42h post infection. During necropsy, milk curd in stomach, fluid in thinwalled small intestines, and ectocolon were observed. During histopathological examination, slight to moderate vil lous stunting were observed. Bulging and sloughing of villous epithelial cells were prominent. Neutrophils were clustered in the lamina propria at villous tips subjacent to disrupted epithelium. In severity segment of villous destruction, necrosis and sloug hing of exposed lamina propria resulted in complete loss of villous strcture.关键词
猪轮状病毒/培养特性/人工感染/致病性Key words
porcine rotavirus/ cell culture,/experimental infection/pathogenicity分类
医药卫生引用本文复制引用
黄小波,徐璐,曹三杰,文心田..猪轮状病毒OSU株的培养特性与致病性研究[J].中国人兽共患病学报,2012,28(2):120-123,147,5.基金项目
国家高技术研究发展计划(863计划)(2009AA10Z402) （863计划）
国家农业部公益性行业(农业)科研(201203056) （农业）
教育部《长江学者和创新团队发展计划》创新团队项目(IRTO848)联合资助 （IRTO848）
