中国人兽共患病学报2012，Vol.28Issue(3)：205-208,4.
贝氏隐孢子虫鸡胚培养方法研究
Cryptosporidium baileyi oocysts cultured in chicken embryo
摘要
Abstract
The aim of the present study was to establish a the chicken embryo culture method acquiring a large number of Cryplosporidium baileyi oocysts for anti-Cryptosporidium drug screening and its vitro culture, in which some conditions containing the effect of temperature, inoculum dose, excystation and recovery time were considered. The 9-day-old chicken embryos were innoculated with 3×105 oocysts in allantoic cavity and incubated for a further 168 hours at 37℃, 38℃ and 40℃ , respectively, in which the number of reproduction of oocysts was not significant different(P>0. 05). Innoculated with 3×105 oocysts the reproduction rates of oocysts was similar compared with innoculated with 1×106oocysts(P>0. 05) and innoculated with 3 ×105 oocysts treated by 0. 75% synthetic sodium taurocholate the number of reproduction of oocysts was not significant different compared with innoculated with 3×105 oocysts treated by 0. 75% synthetic sodium taurocholate(P>0. 05). But the number of reproduction of oocysts incubated for a further 168h was more than incubated for a further 144h and 192h(P<0. 05). The conclusions were that recovery time was vital factor in collecting more C. Baileyi oocysts. Oocysts excystation was unnecessary and it was suitable that chicken embryos innoculate with 3×105 oocysts at 40℃.关键词
贝氏隐孢子虫/鸡胚/体外培养Key words
Cryplosporidium baileyi/chicken embryo/in vilro culture分类
医药卫生引用本文复制引用
黄磊,张云,张素梅,菅复春,宁长申,张龙现..贝氏隐孢子虫鸡胚培养方法研究[J].中国人兽共患病学报,2012,28(3):205-208,4.基金项目
教育部博士点基金项目(No.20094105110003)和国家自然科学基金项目(No.30871863)联合资助 （No.20094105110003）
