中国人兽共患病学报2012，Vol.28Issue(3)：230-236,7.
诺卡菌的培养和染色特征研究
Characteristics of cultivation and staining of Nocardia species
摘要
Abstract
To investigate the cultivation and staining characteristics of Nocardia , 3 different media including Brain Heart Infusion Agar, Columbia Blood Agar Base and Sabouraud Dextrose Agar were used for incubating 23 Nocardia reference strains. The growth status, time and colony morphology of Nocardia at 25°C and 37°C were observed. The culture features and stain characteristics were observed by means of Gram's stain, Kinyoun acid-fast stain, and modified Kinyoun acid-fast methods. Total of 7 species including 23 strains from DSMZ (Germany) were observed. Colonial morphology and colour of Nocardia were variable. The colonial morphology was needle-shaped, snow-shaped, cauliflower-shaped and irregular-shaped appearance. Nocardia colonies varied from white to yellow, orange and grey in colour. Nocardia species were gram positive and weakly acid-fast bacteria. The higher the positive rate of modified acid-fast staining, the lower the concentration of decol-ourizer,when using different concentration of sulfuric acid as decolourizer . The positive rate was the highest, when using 0. 125% sulfuric acid for decolourization. Nocardia species have special cultivation features and unusal staining characteristics.关键词
诺卡氏菌/培养/染色/改良抗酸染色Key words
Nocardia/culture/stain/modified Kinyoun acid-fast stain分类
医药卫生引用本文复制引用
张媛,张媛媛,万康林,李振军..诺卡菌的培养和染色特征研究[J].中国人兽共患病学报,2012,28(3):230-236,7.基金项目
国家自然科学基金项目(No.30800029)资助 （No.30800029）
