中国人兽共患病学报2012，Vol.28Issue(3)：244-247,251,5.
肠炎血清型沙门菌多重PCR检测方法的建立及应用
Development of a multiplex PCR assay for the identification of Salmonella enterica serovar enteritidis
摘要
Abstract
In order to develop a rapid method for the detection of the prevalent Sahnonella enierica serovar enteritidis, a multiplex PCR method was developed and validated. Portions of the gene sequences of somatic antigen for Salmonella sero-groups A/Dl, flagellar antigens for fliC-HG and Salmonella difference fragment (sdfl) were targeted for amplification using four primer pairs. The multiplex PCR was developed and optimized. To valid the assay, genomic DNA from 14 Sahnonella strains representing 14 serotypes and 18 non-salmonella strains was subjected to the PCR. The method was applied to the detection of 53 samples isolated from 2009 to 2010 in Zhejiang Province. The results showed the four targeted genes were correctly amplified only from Salmonella enieriiidis. The assay could differentiate Salmonella serogroup A from Salmonella sero-group D1 , and it also could identify the Salmonella with fliC-Hc. The coincidence rate of the actual sample detection was up to 100%. It is concluded that this multiplex PCR method can identify Salmonella enieriiidis enterica serovars directly. It can make up for deficiencies of uneven quality of commerical serum. It should be an assistant method to the traditional serotyping method. It is a simple, rapid, reliable and reproducible method of Sahnonella enieriiidis detection that will aid in surveillance, prevention and control of this pathogen.关键词
多重PCR/肠炎沙门菌/血清型/sdf I基因Key words
multiplex PCR/Salmonella enieriiidis/serovar/Salmonella difference fragment分类
医药卫生引用本文复制引用
占利,叶菊莲,梅玲玲,程苏云,罗芸,杨婷婷..肠炎血清型沙门菌多重PCR检测方法的建立及应用[J].中国人兽共患病学报,2012,28(3):244-247,251,5.基金项目
浙江省钱江人才科技计划(No.2007R10018) （No.2007R10018）
