中国人兽共患病学报2012，Vol.28Issue(3)：256-260,283,6.
MLVA方法在军团菌分子分型中的应用评价
Assessment on the application of multiple-locus variable-number tandem repeat analysis for Legionella subtyping
摘要
Abstract
To evaluate the applicability of multiple-locus variable-number tandem repeat analysis for Legionella subtyp-ing, 9 VNTR loci were selected to type 81 Legionella pneumophila serogroup 1 strains isolated from environmental water in China. There were 6, 3, 4, 2, 3, 3, 2, 4 and 7 different products amplified from 9 VNTR loci respectively. No specific amplification was obtained from several loci of some strains. Repeated sequences were identified in PCR products of 7 loci. No repeated sequence was found in all sequenced products from locus Lpms3 and in some products from locus Lpms31. The sequences of tandem repeats varied within the same isolate as among isolates. Incomplete tandem repeats were found in 3 loci. Based on the polymorphisms of 8 loci, 81 Lp1 strains could be classified into 19 types. Comparatively, these strains could be divided into 4 6 species and 21 molecular types by PFGK and SBT respectively. So, MLVA was less sensitive for Legionella subtyping. But it was easy and low-cost, and could be used in relatively simply equipped laboratories.关键词
军团菌/嗜肺军团菌/分子分型/MLVAKey words
Legionella/Legionella pneumophila/molecular typing/MLVA分类
医药卫生引用本文复制引用
朱兵清,任红宇,周海健,关红,邵祝军..MLVA方法在军团菌分子分型中的应用评价[J].中国人兽共患病学报,2012,28(3):256-260,283,6.基金项目
"十一五"科技重大专项(2008ZX10004-007,2008ZX10004-008) （2008ZX10004-007,2008ZX10004-008）
