中国人兽共患病学报2012，Vol.28Issue(3)：274-277,4.
云南某农村猪戊型肝炎病毒分子流行病学调查
Molecular epidemiology of swine HEV in a village of Yunnan
摘要
Abstract
To investigate the prevalence of swine HKV in rural areas of Yunnan province, 78 swine stool samples were collected from a village of Yunnan for HEV ORF2 detection by Reverse Transcription-nested PCR (RT-nested PCR). The PCR products were detected by electrophoresis on agarose gel and purified, and then cloned into pMD18-T vector and sequenced. The homology and phylogenetic tree were analyzed by DNAStar and MEGA 4.0, respectively. Results indicated that the expected 350 bp fragments were obtained, which shared 77. 4%- 97. 4% homologies with different HEV genotypes, 97. 4% with genotype 4 and 77. 4% with genotype 3. Phylogenetic analysis suggested that the HEV isolates in this study were clustered together with genotype 4 reference isolates, which means these isolates belonged to genotype 4. In the present study, 8 samples were positive to swine HEV, and the positive rate was 10. 26% (8/78), which indicated a high risk of HEV infection to humans through the zoonotic transmission.关键词
猪戊型肝炎病毒/逆转录巢式PCR/分子流行病学/调查Key words
swine hepatitis E virus/RT-nested PCR/molecular epidemiology/investigation分类
医药卫生引用本文复制引用
黄芬,禹文海,马天武,井申荣,曾韦锟..云南某农村猪戊型肝炎病毒分子流行病学调查[J].中国人兽共患病学报,2012,28(3):274-277,4.基金项目
云南省应用基础研究面上项目(2009ZC184M)、云南省教育厅基础研究面上项目(2011Y382)、云南省应用基础研究面上项目(2011FZ068)联合资助,黄芬和禹文海为同等贡献 （2009ZC184M）
