中国人兽共患病学报2012，Vol.28Issue(3)：278-283,6.
婴幼儿疑似甲型H1N1重症病例及门诊流感样病例中鼻病毒的检测分析
Detection and analysis on rhinovirus in children with severe influenza A (H1N1/2009)-like infection and influenza-like illness
摘要
Abstract
The aim of the present research was to study the infection, prevalence and evolution of human rhinovirus in children with severe influenza A (HlNl/2009)-like infection and influenza-like illness, and to study the coinfection for HRV with the other respiratory viruses. Throat swab/endotracheal aspirates specimens were collected from 24 6 children with severe influenza A (HlNl/2009)-like infection (group 1), and throat swab specimens were collected from 68 children with influenza-like illness (group 2). Molecular tests were used to detect its 5' noncoding region and VP2-VP4 of human rhinovirus. Nucleic acid sequence for VP2-VP4 was used for phylogenetic reconstruction. The co-infection for HRV with the other respiratory viruses was analysed. Results showed that in the 246 specimens of group 1, 21 specimens were HRV positive (8. 54%). In the 68 specimens of group 2, 11 specimens were HRV positive (16. 2%). The rate of co-infection for HRV with another viruses was 71. 4 % and 9. 09 % in group 1 and group 2, respectively, which had significant difference (P<0. 05). Among the 14 specimens which were sequenced successfully, the rate for 3 HRV species, HRV-A, HRV-B, and the novel species of HRV-C, was 64. 3% , 7. 1% , and 28. 6% , respectively. There were only 55% to 65% nucleotide acid identity among the 3 HRV genotypes. In conclusion, the main infection is coinfection of HRV in severe influenza A (HlNl/2009)-like illness. The main genotypes of human rhinovirus in children with severe influenza A (H1N1/2009) -like infection and with influenza-like illness are HRV-A and HRV-C in Hangzhou. The nucleotide acid of HRVs are quite variable.关键词
鼻病毒/甲型H1N1流感/流感样病例/合并感染Key words
rhinovirus/influenza A (H1N1/2009)/influenza-like illness/coinfection分类
医药卫生引用本文复制引用
于新芬,潘劲草,寇宇,李品艳,叶榕,周银燕..婴幼儿疑似甲型H1N1重症病例及门诊流感样病例中鼻病毒的检测分析[J].中国人兽共患病学报,2012,28(3):278-283,6.基金项目
杭州市医疗卫生科研项目计划(20110833B36) （20110833B36）
