中国水产科学2012，Vol.19Issue(1)：45-50,6.DOI:10.3724/SP.J.1118.2012.00045
去眼柄后中华绒螯蟹法呢酸甲基转移酶表达和卵巢发育
FAMeT expression and ovarian development following eyestalk ablation in Chinese mitten crab (Eriocheir sinensis)
摘要
Abstract
We evaluated the role of methyl famesoate (MF) during ovarian development in Eriocheir sinensis. We cultured oocytes with mandibular organ (MO)as group 1, and oocytes with MO and X-organ-sinus gland (XO-SG) as group 2 in vitro. Oocytes cultured with medium were set as control. MO excretion promoted oocyte maturation by increasing the oocyte diameter at the 1st, 3rd, 6th, and 14th day after eyestalk ablation, particularly on the 6th day (P<0.0l). Incubation with XO-SG significantly inhibited oocyte development (P<0.05). We used fluorescent quantitative RT-PCR to quantify FAMeT expression. Levels were 265% higher than 6 and 14 days after eyestalk ablation, and were significantly higher than that in the control group (without MO) (P<0.01). FAMeT expression levels were lower on day 14 than that on day 6 and there was no change on day 1 and day 3 after eyestalk ablation (P>0.05). Our results suggest that the functional material in MO induces oocyte maturation, with the effect being strongest 6 days after eyestalk ablation. MO FAMeT mRNA was downregulated by the XO-SG causing inhibition of oocyte maturation in vitro.关键词
中华绒螯蟹/法呢酸甲基转移酶/大颚器/卵巢发育/组织培养/去眼柄分类
农业科技引用本文复制引用
郭敏,魏华,陶贤继,吴婷婷..去眼柄后中华绒螯蟹法呢酸甲基转移酶表达和卵巢发育[J].中国水产科学,2012,19(1):45-50,6.基金项目
上海市水生生物重点学科建设项目(S30701) （S30701）
上海市现代农业产业技术体系建设(中华绒螯蟹,D-8003-10-0208) （中华绒螯蟹,D-8003-10-0208）
上海高校创新团队项目(第二期). （第二期）
