中国水产科学2012，Vol.19Issue(1)：51-61,11.DOI:10.3724/SP.J.1118.2012.00051
温度与盐度对吉富品系尼罗罗非鱼幼鱼能量代谢的联合效应
Combined effect of temperature and salinity on energy metabolism of GIFT Nile tilapia (Oreochromis niloticus) juveniles
摘要
Abstract
We evaluated the combined effects of temperature and salinity on the energy metabolism of Nile tilapia juveniles (Oreochromis niloticus, GIFT strain). We used a two-factor central composite experimental design (CCD) and response surface methodology (RSM) to test the effects of temperature (13-36°C) and salinity (0-20) on the oxygen consumption rate (OR), ammonia excretion rate (NR), and CO2 excretion rate (CR) under laboratory conditions. The linear effects of temperature and salinity, the quadratic effect of salinity, and the interaction between salinity and temperature had significant effect on OR, NR and CR (PO.05). The quadratic effect of temperature was not associated with changes in OR and CR (P>0.05). The model equations for OR, NR, and CR had R2 values of 0.958 1, 0.977 8, and 0.979 5, respectively (P<0.01), and could be used for prediction. The OR, NR, and CR were influenced more by temperature than by salinity. At lower salinities, energy metabolism was sensitive to changes in temperature, and OR, NR, and CR were higher around the iso-osmotic point. However, when salinity exceeded the iso-osmotic point, lower temperatures and higher salinity inhibited metabolism. The O : N ratio did not vary with temperature, but vary with salinity (P>0.05). Within the temerature range of 13-36°C and salinity range of 0-10, tilapia juveniles utilized protein-lipid- dominated metabolism. Conversely, the juveniles depended on lipid-dominated catabolism at salinity of 20. The mean respiratory quotient value was 0.752. Our results provide insight into the joint influence of temperature and salinity on the metabolism of tilapia juveniles and provides a bioenergetic basis for the study of how environmental factors govern the spatial and temporal pattern of energy distribution in this species.关键词
尼罗罗非鱼/幼鱼/耗氧率/排氨率/CO2排泄率Key words
Oreochromis niloticus/ juvenile/ oxygen consumption rate/ ammonia excretion rate/ CO2 excretion rate分类
农业科技引用本文复制引用
王辉,强俊,王海贞,徐跑,李瑞伟..温度与盐度对吉富品系尼罗罗非鱼幼鱼能量代谢的联合效应[J].中国水产科学,2012,19(1):51-61,11.基金项目
国家科技支撑计划项目(2008BADB9B01-3) （2008BADB9B01-3）
农业部公益性行业科研专项(200903046-02) （200903046-02）
广东省教育部产学研结合项目(2008B090500088,2010B090500032). （2008B090500088,2010B090500032）
