中国水稻科学2011，Vol.25Issue(6)：599-604,6.DOI:10.3969/j.issn.1001-7216.2011.06.006
sbk和sck双价抗虫转基因水稻的育成和鉴定
Breeding and Identification of Insect-Resistant Rice with Two Insecticidal Genes, sbk and sck
摘要
Abstract
The plasmid of pCDMARUBA-Hyg, which contained two insect-resistant genes, sbk\_modified from CryiA(c)] and sck(modified from CpTI), was transformed into an Agrobacterium EHA105 for infection of the calli of a super japonica rice Nanjing 45. Plants regenerated from the transformation were subjected to all kinds of gene verifications and selection. Using PCR detection with the primers of sbk and sck genes. 42 positive transgenic plants that were marker-free and contained the two target genes were selected from 97 regenerated plants. Results of Southern-blotting indicated that 23, 11, 5, 2 and 1 plants had one. Two, three, four and five copies of the transformed genes, respectively. Analyses of RT-PCR and Bt gene testing paper showed that 28 T3 generation plants derived from four transgenic plants having a single copy were insect-resistant. Feeding experiment with rice stem borer revealed that the insect resistance was greatly increased with nymph mortality ranging from 94% to 100%. In addition, among the transgenic plants, three T3 transgenic plants had many desirable characteristics for production. The mechanism of insect-resistance of Bt gene and its application in rice transgenic research were also briefly discussed.关键词
水稻/sbk基因/sck基因/抗虫/转基因/育种Key words
Oryza sativa / sbk gene/ sck gene/ insect resistance/ transgene/ breeding分类
农业科技引用本文复制引用
李聪,张启军,刘少奎,赖东,漆庆明,吕川根..sbk和sck双价抗虫转基因水稻的育成和鉴定[J].中国水稻科学,2011,25(6):599-604,6.基金项目
江苏省自然科学基金资助项目(BK2008348) （BK2008348）
国家转基因生物新品种培育科技重大专项(2008ZX08001-004). （2008ZX08001-004）
