首页|期刊导航|中国神经再生研究（英文版）|Protein expression of sensory and motor nerves Two-dimensional gel electrophoresis and mass spectrometry
中国神经再生研究（英文版）2012，Vol.7Issue(5)：369-375,7.DOI:10.3969/j.issn.1673-5374.2012.05.008
Protein expression of sensory and motor nerves Two-dimensional gel electrophoresis and mass spectrometry
Protein expression of sensory and motor nerves Two-dimensional gel electrophoresis and mass spectrometry
摘要
Abstract
The present study utilized samples from bilateral motor branches of the femoral nerve, as well as saphenous nerves, ventral roots, and dorsal roots of the spinal cord, to detect differential protein expression using two-dimensional gel electrophoresis and nano ultra-high performance liquid chromatography electrospray ionization mass spectrometry tandem mass spectrometry techniques. A mass spectrum was identified using the Mascot search. Results revealed differential expression of 11 proteins, including transgelin, Ig kappa chain precursor, plasma glutathione peroxidase precursor, an unnamed protein product (gi|55628), glyceraldehyde-3-phosphate dehydrogenase-like protein, lactoylglutathione lyase, adenylate kinase isozyme 1, two unnamed proteins products (gi|55628 and gi|1334163), and poly(rC)-binding protein 1 in motor and sensory nerves. Results suggested that these proteins played roles in specific nerve regeneration following peripheral nerve injury and served as specific markers for motor and sensory nerves.关键词
differential protein expression/mass spectrometry/motor nerve/peripheral nerve-specific regeneration/two-dimensional gel electrophoresis/sensory nerveKey words
differential protein expression/mass spectrometry/motor nerve/peripheral nerve-specific regeneration/two-dimensional gel electrophoresis/sensory nerve引用本文复制引用
Zhiwu Ren,Yu Wang,Jiang Peng,Li Zhang,Wenjing Xu,Xiangdang Liang,Qing Zhao,Shibi Lu..Protein expression of sensory and motor nerves Two-dimensional gel electrophoresis and mass spectrometry[J].中国神经再生研究（英文版）,2012,7(5):369-375,7.基金项目
The study was supported by the Key Projects in the National Science & Technology Pillar Program,No.2009BAI87B02 （）
the National Natural Science Foundation of China,No.31100696 （）
the National Basic Research Program of China (973 Program),No.2012CB518106. （973 Program）
