中国神经再生研究（英文版）2012，Vol.7Issue(9)：708-713,6.DOI:10.3969/j.issn.1673-5374.2012.09.012
Recombinant adeno-associated virus delivered human thioredoxin-PR39 prevents hypoxia-induced apoptosis of ECV304 cells
Recombinant adeno-associated virus delivered human thioredoxin-PR39 prevents hypoxia-induced apoptosis of ECV304 cells
Xiyun Ruan 1Zhenguo Yuan 2Yifeng Du 1Guangxiao Yang 3Quanying Wang3
作者信息
- 1. Department of Neurology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong Province, China
- 2. Shandong Medical Imaging Research Institute, Affiliated to Shandong University, Jinan 250021, Shandong Province, China
- 3. Xi'an Huaguang Biological Engineering Co., Ltd., Xi'an 710025, Shaanxi Province, China
- 折叠
摘要
Abstract
Human thioredoxin and antibacterial peptide, PR39, have been shown to have potent antioxidant effects that may prolong survival of cells during hypoxia. The pSSCMV/human thioredoxin-PR39 vector was successfully constructed in this study and used to infect ECV304 cells. Transfected ECV304 cells were incubated at 1%, 5% hypoxic, and normal oxygen conditions. We found that the number of apoptotic cells after transfection with recombinant adeno-associated virus-human thioredoxin -PR39 was significantly lower than controls, suggesting a protective effect of the recombinant human thioredoxin-PR39 protein on hypoxic cells.关键词
human thioredoxin/antimicrobial peptide PR39/fusion gene/recombinant adeno-associated virus/gene therapy/apoptosis/hypoxiaKey words
human thioredoxin/antimicrobial peptide PR39/fusion gene/recombinant adeno-associated virus/gene therapy/apoptosis/hypoxia引用本文复制引用
Xiyun Ruan,Zhenguo Yuan,Yifeng Du,Guangxiao Yang,Quanying Wang..Recombinant adeno-associated virus delivered human thioredoxin-PR39 prevents hypoxia-induced apoptosis of ECV304 cells[J].中国神经再生研究（英文版）,2012,7(9):708-713,6.
