中国生物防治学报2011，Vol.27Issue(4)：458-463,6.
异色瓢虫乳酸脱氢酶基因的克隆与序列分析
Cloning and Sequence Analysis of Lactic Dehydrogenase Gene from Harmonia axyridis （Coleoptera： Coccinellinae）
摘要
Abstract
Lactic dehydrogenase （LDH） of Harmonia axyridis was cloned by homological cloning and anchored PCR. The full length of HaxLDH cDNA sequence is 1336 bp （GenBank accession number HM362898）, which contains a 3＇ untranslated region of 214 bp and a 5＇ untranslated region of 123 bp. HaxLDH cDNA contains an open reading frame of 999 bp, encoding a protein of 332 amino acids residues with calculated molecular mass of 36.20 kD and pI of 8.62. It has one potential N-glycosylation site （residue 352）, with no signal peptide, putative cleavage sites and transmembrane domain. Blast analysis indicated that HaxLDH contains eight conservative domains, DLQHG, TAGV/ARQK/RE/DGES/TRL, LVQRN, GSGTNLD, SCHGW/YI/VINGEHGD, VWSG/AVNV/IAGV, AYEV/IIK/RLKGYTS/NWAI/VGLS and LSLP of LDH family. HaxLDH had 67% identity with that of Tribolium castaneum, and phylogenetic analysis shows that these two species are most closely related.关键词
异色瓢虫/乳酸脱氢酶/基因克隆/序列分析Key words
Harmonia axyridis/lactic dehydrogenase/gene cloning/sequence analysis分类
农业科技引用本文复制引用
王丹卉,秦资,韩国斌,王世贵,唐斌,张帆..异色瓢虫乳酸脱氢酶基因的克隆与序列分析[J].中国生物防治学报,2011,27(4):458-463,6.基金项目
国家“973”计划项目（2009CBll9206,2006CBl02005-3) （）
国家自~（31071731,31000880) （）
杭州市科技局计划项目（20091133808)~浙江省大学生科技创新活动计划（新苗人才计划)：杭州师范大学生物科学国家特色专业本科生科技创新项目 （）
杭州师范大学优秀中青年教师支持计划（JTAS2011.叭.031) （）
