中国生物防治学报2011，Vol.27Issue(4)：504-509,6.
枯草芽孢杆菌XF-1中脱乙酰几丁质酶在大肠杆菌中的表达及其抑茵活性
Expression of Chitosanase Gene of Bacillus subtilis XF-1 in Escherichia coli and the Chitosanase Antagonism against the Fungal Pathogens
摘要
Abstract
Chitosanase gene （csn） of Bacillus subtilis XF-1, which was amplified with primers designed according to its reported homologous sequence, was cloned and sequenced. The gene had an 83 lbp coding sequence, encoding a protein with 30 kD molecular weight composed of 277 amino acids. Similarity of XF-1 csn sequence with that of B. subtilis 168 was 99% and homolog of amino acid sequence was 98%. The differences of amino acid sequence from that of B168 appeared in the 19th, 47th, 60th, 256th and 257th amino acids, and the five amino acids of methionine, glutamic acid, isoleucine, glutamic acid, asparagine were replaced with isoleucine, valine, threonine, aspartic acid and lysine, respectively. The XF- 1 strain was inhibitory to Magnaporthe oryzae and Plasmodiophora brassicae, but B168 was not. The expressed proteins of csn from XF-1 and B168 in Escherichia coli both had chitosanase activity, but the former had inhibitory effect on M. oryzae and P. brassicae compared with the latter. Theresults indicated that chitosanase is one of mechanisms of XF-1 against P. brassicae and the replacements of 5 amino acids above may contribute to its antimicrobial activity.关键词
枯草芽孢杆菌XF－1/脱乙酰几丁质酶/根肿菌/抑菌作用Key words
Bacillus subtilis XF-1/chitosanase/Plasmodiophora brassicae/antagonism分类
农业科技引用本文复制引用
赵静,熊国如,王志远,毛自朝,吴毅歆,何月秋..枯草芽孢杆菌XF-1中脱乙酰几丁质酶在大肠杆菌中的表达及其抑茵活性[J].中国生物防治学报,2011,27(4):504-509,6.基金项目
科技部国际科技合作项目（2009DFA32360) （）
农业部公益性行业（农业)科研专项（201003029) （）
云南省自然科学基金重点项目（2008CC024) （）
云南省科技强省专项（2009EB060) （）
云南省教育厅科学研究基金项目（09J0073) （）
