中国生物防治学报2011，Vol.27Issue(4)：520-527,8.
响应曲面法优化利迪链霉菌A02发酵培养基
Optimization of Medium Components for Production of Natamycin by Streptomyces lydicus A02 with Response Surface Methodology
摘要
Abstract
In order to increase the yield ofnatamycin produced by Streptomyces lydicus A02, fermentation medium was optimized with methods of fractional factorial design, path of steepest ascent and central composite design. Results showed that corn starch, cotton seed flour and glucose were the major factors affecting natamycin production. According to the evaluation by the response surface model established in this study, the optimum mass concentrations of the three components in fermentation medium were 52.7, 31.7 and 11.7 g.L- 1, respectively, with the highest natamycin yield of 1735.3 mg.L-J. Fermentation experiments with shake flasks and 30 L fermentor verified that there was no statistical difference between the yield value forecasted by the model and that obtained in the tests, and the natamycin production was increased by 32.9%--34.8% in the optimized medium in comparison with basic fermentation medium. This result indicated that the optimal formula of the medium for natamycin production of S. lydicus established with response surface methodology was applicable and effective, and it will provide reliable data for design of industrial medium and establishment of fermentation process for natamycin produced by S. lydicus.关键词
利迪链霉菌/纳他霉素/发酵培养基/响应面设计Key words
Streptomyces lydicus/natamycin/fermentation medium/response surface methodology分类
农业科技引用本文复制引用
花玉鹏,文才艺,刘伟成,杨剑芳,卢彩鸽..响应曲面法优化利迪链霉菌A02发酵培养基[J].中国生物防治学报,2011,27(4):520-527,8.基金项目
北京市科技计划（Z101105052010002) （）
公益性行业（农业)科研专项（200903049-07)：北京市农林科学院项目（2010A006) （）
