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转crylA基因水稻和两种杀虫剂对非靶标天敌拟环纹豹蛛免疫影响的评价

田俊策 陈洋 李兆亮 彭于发 叶恭银

中国生物防治学报2011,Vol.27Issue(4):559-563,5.
中国生物防治学报2011,Vol.27Issue(4):559-563,5.

转crylA基因水稻和两种杀虫剂对非靶标天敌拟环纹豹蛛免疫影响的评价

Assessment of Effect of Transgenic Rice with crylAb Gene and Two Insecticides on Immune of Non-target Natural Enemy, Pardosa pseudoannulata

田俊策 1陈洋 1李兆亮 1彭于发 2叶恭银1

作者信息

  • 1. 浙江大学昆虫科学研究所/水稻生物学国家重点实验室/农业部作物病虫分子生物学重点开放实验室,杭州310029
  • 2. 中国农业科学院植物保护研究所植物病虫生物学国家重点实验室,北京100193
  • 折叠

摘要

Abstract

The immune parameters (total number of hemocytes, encapsulation rate and phenoloxidase activity) of Pardosapseudoannulata from the transgenic crylAb rice (KMD1 and KMD2) fields and non-transgenic parental rice variety (Xiushui 11) fields which were sprayed the insecticides (imidacloprid and fipronil) were measured and compared to those parameters measured in spiders collected from Xiushui 11 with no insecticide sprayed. Results indicated that there were no significant differences in the immune factor of the P pseudoannulata between Bt rice and the non-insecticide sprayed Xiushui i1. However, insecticides (imidacloprid and fipronil) reduced phenoloxidase activity and significantly reduced the total number of hemocytes and encapsulation rate of/3. pseudoannulata. Thus, it was clear that two insecticides had adverse effects on the immune response of P pseudoannulata. Therefore, immune factors of non-target natural enemies could be considered to be a simple and efficient indicator in the ecological safety assessment of transgenic rice.

关键词

转基因水稻/拟环纹豹蛛/免疫指标

Key words

transgenic rice/Pardosa pseudoannulata/immune factor

分类

生物科学

引用本文复制引用

田俊策,陈洋,李兆亮,彭于发,叶恭银..转crylA基因水稻和两种杀虫剂对非靶标天敌拟环纹豹蛛免疫影响的评价[J].中国生物防治学报,2011,27(4):559-563,5.

基金项目

国家重大基础研究发展计划(973)(2007CBl09202) ()

国家转基因生物培育重大专项(2008ZX08011-01) ()

中国生物防治学报

OA北大核心CSCDCSTPCD

2095-039X

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