中国生物防治学报2012，Vol.28Issue(1)：87-94,8.
利用编码harpin蛋白的基因遗传改良生防荧光假单胞菌2P24
Genetic Modification of a Biocontrol Agent Pseudomonas fluorescens 2P24 by a Harpin Coding Gene
摘要
Abstract
Biocontrol agent Pseudomonas fluorescens 2P24,one of plant growth-promoting rhizobacteria（PGPR）,possesses a type-Ⅲ secretion system（T3SS） for association with plants.To enable 2P24 to express a harpin protein which triggers hypersensitive response（HR） in plants and to have the ability to induce resistance to plant pathogen infection,we constructed a fusion of a hap1 gene,which encodes a harpin of Xanthomonas oryzae pv.oryzicola,with a promoter of avrPto1 of Pseudomonas syringae pv.tomato DC3000 which was induced in 2P24.This recombination led 2P24 to express the harpin protein Hpa1.The protein,secreted via the T3SS of 2P24,triggered HR in tobacco via the activation of HR marker genes,HIN1 and HRS203J,and pathogenesis-related protein gene PR1a.The genetic modified 2P24 with the harpin coding gene,as the recipient strain 2P24,had antimicrobial ability against wheat scab Fusarium graminearum and cotton wilt F.oxysporum f.sp.vasinfectum.Using HR-elicitors from plant-pathogen interactions to genetically modify biocontrol antimicrobes provides a fundamental basis for plant disease control.关键词
荧光假单胞菌/harpin/过敏反应/遗传改良Key words
Pseudomonas fluorescens/harpin/hypersensitive response/genetic modification分类
农业科技引用本文复制引用
赵倩,邹丽芳,邹华松,李玉蓉,陈功友..利用编码harpin蛋白的基因遗传改良生防荧光假单胞菌2P24[J].中国生物防治学报,2012,28(1):87-94,8.基金项目
国家自然科学基金(31071656) （31071656）
国家"863"计划(2006AA10A210) （2006AA10A210）
