中国输血杂志2011，Vol.24Issue(12)：1044-1047,4.
不同核酸检测方法对献血者隐匿性乙肝病毒感染的研究
Study of occult hepatitis B virus infection among blood donors by different NAT methods
摘要
Abstract
Objective To study occult hepatitis B virus infection (OBI) in blood donors and compare the capability of detecting OBI by different NAT methods. Methods Procleix Ultrio automatic assay system and nested-PCR were used to detect HBV infection in the plasma samples of blood donors. NAT-reactive samples were subjected to HBV DNA sequence analysis. Results A total of 9 209 samples were assayed,in which 50 were HBsAg positive and 9 159 HBsAg negative. In HBsAg negative samples,18 were tested HBV DNA positive by nested-PCR,whereas 7 were tested HBV DNA positive by Procleix Ultrio. The results of HBV DNA sequence analysis indicated that genotype C had a higher proportion in OBI cases than in HBsAg positive cases(64.1% vs 23.1% ,P <0.01 ). Conclusion There is a high proportion of occult hepatitis B virus infection in blood donors in south Fujian China,and there was divergence in the capability of detecting OBI by different NAT methods.关键词
献血者/闽南地区/核酸检测/血液筛查/隐匿性乙肝病毒感染Key words
NAT/ Blood Screening/ OBI/ Blood Donor分类
医药卫生引用本文复制引用
倪宏英,欧山海,谢金镇,林永财,陈长荣..不同核酸检测方法对献血者隐匿性乙肝病毒感染的研究[J].中国输血杂志,2011,24(12):1044-1047,4.基金项目
福建省医学创新课题(2009-CXB-71) （2009-CXB-71）
