中国实验动物学报2011，Vol.19Issue(6)：461-464,4.DOI:10.3969/j.issn.1005-4847.2011.06.003
人载脂蛋白A1基因转基因小鼠的建立
Establishment of a human APOA1 transgenic mouse model
摘要
Abstract
Objective To establish a human AP0A1 transgenic mouse model. Methods The transgenic plasm id was constructed by inserting human AP0A1 gene into the downstream of the human ubiquitin C ( Ubc) promoter. The transgenic mice were produced by microinjection and the genotyping was detected by PCR. The expression level of the gene was determined by Western blotting. The levels of blood lipids of the mice of different ages were detected using a biochemical analyzer. Results Two human AP0A1 gene transgenic mouse lines with different expression levels were established. The expression levels of human AP0A1 gene in blood, liver, heart, kidney, spleen and blood vessels of the transgenic mice were significantly higher than those from wild type mice. The blood biochemical assay showed that the level of HDL-cholesterol in the transgenic mice was significantly higher and the level of TG was lower than that of the wild type mice. Conclusions The transgenic mice with overexpression of human APOA1 gene have been successfully established. It may be a useful animal model for further research of hyperlipidemia and related angiocardiopathy.关键词
载脂蛋白A1/转基因小鼠/高密度脂蛋白胆固醇Key words
Apolipoprotein Al/ Transgenic mice/ High-density lipoprotein cholesterol分类
生物科学引用本文复制引用
张旭,高昆,张海涛,高翔,葛文平,张小娟,张连峰,董伟..人载脂蛋白A1基因转基因小鼠的建立[J].中国实验动物学报,2011,19(6):461-464,4.基金项目
卫生部:实验动物和人类疾病动物模型资源扩展(200802036). （200802036）
