中国实验动物学报2011，Vol.19Issue(6)：512-516,5.DOI:10.3969/j.issn.1005-4847.2011.06.012
耐甲氧西林金黄色葡萄球菌诱导SKH-1无毛小鼠菌血症模型
Establishment of a SKH-1 hairless mouse model of bacteraemia induced by MRSA infection
摘要
Abstract
Objective To establish a mouse model of bacteraemia infected with methicillin resistant Staphylococcus aureus (MRSA) and its evaluation system. Methods Sixty-two specific pathogen free SKH-1 hairless mice were randomly divided into 4 groups: the PBS,low-dose infection, high-dose infection and drug-treated infection groups. Mice were challenged with MRSA (ST-239) by intravenous tail injection. The bacteraemia models were evaluated by body weight, mortality, blood tests, blood bacterial concentrations, organs infection measuring and pathological examination, and the models were also verified with antibiotic teicoplanin. Results The infected mice had evident weight loss, high mortality, sharp changes in blood tests, high bacterial concentration in the blood and pathological alterations in many organs sush as in the liver and kidney. Teicoplanin reduced the mortality markedly and alleviated lesions of organs. Conclusions A SKH-1 hairless mouse model of bacteraemia has been successfully established by MRSA infection. This mouse model has apparent pathological features, is easy to be observed, and provides an ideal animal model for MRSA-related drugs evaluation studies.关键词
耐甲氧西林金黄色葡萄球菌/菌血症/模型/SKH-1无毛小鼠Key words
methicillin resistant Staphylococcus aureus, MRSA/ Bacteraemia/ Model/ SKH-1 hairless mice分类
生物科学引用本文复制引用
陈雪雨,彭秀华,杨玉琴,周文江,周光兴..耐甲氧西林金黄色葡萄球菌诱导SKH-1无毛小鼠菌血症模型[J].中国实验动物学报,2011,19(6):512-516,5.基金项目
上海市科技发展基金项目(编号:09140900700). （编号:09140900700）
