中国实验动物学报2011，Vol.19Issue(6)：517-521,5.DOI:10.3969/j.issn.1005-4847.2011.06.013
镉染毒大鼠胫骨骨微结构的活体Micro-CT观察
Micro-CT analysis of the tibia microstructure in live male rats exposed to cadmium
秦波音 1陈晓 2朱国英 2周文江1
作者信息
- 1. 上海市公共卫生临床中心,上海201508
- 2. 复旦大学放射医学研究所,上海200032
- 折叠
摘要
Abstract
Objective To investigate the effects of cadmium on bone microstrueture in live male rats by micro-CT imaging. Methods Twenty-four 3-month-old Sprague-Dawley male rats were randomly divided into 4 groups which were given CdCl2 by subcutaneous injection at a doses of 0 (sodium chloride) , 0. 1 , 0. 5 and 1. 5 mg/kg body weight, respectively. At the 8th week, micro-CT analysis was performed on the proximal tibia for bone microstrueture evaluation. Trabecu-lar bone was reconstructed and selected for analysis within a conforming volume of interest (cortical bone excluded) commencing at a distance of 0. 8 mm from the growth plate, and extending a further longitudinal distance of 1. 2 mm in the distal direction. Results The body weights of cadmium-treated rats were all lower than that in the controls. The volumetric bone mineral density of cadmium-treated rats at the dose of 1. 5 mg/kg bw was significantly lower than that of rats treated with sodium chloride (P<0. 05). Bone microstructure index (bone fraction volume, trabecular number) and trabecular separation of rats treated with cadmium at 1. 5 mg/kg bw were significantly lower and significantly higher than that in the controls ( P < 0. 01) , respectively. Structure model index of cadmium-treated rats was obviously larger than that in the controls. 2-dimensional and 3-dimensional images also revealed the damages of bone microstructure (trabecular conjunction points decreased and dissociated ends increased) caused by cadmium exposure. Conclusions These observations provide an interpretation for cadmium damaging effects on bone from structural viewpoint. Cadmium can induce bone microstructure damage, especially to trabecular bone.关键词
镉/大鼠/骨微结构/Micro-CTKey words
Cadmium/ Rat/ Bone microstructure/ Micro-CT分类
生物科学引用本文复制引用
秦波音,陈晓,朱国英,周文江..镉染毒大鼠胫骨骨微结构的活体Micro-CT观察[J].中国实验动物学报,2011,19(6):517-521,5.
