中国实验动物学报2011，Vol.19Issue(6)：525-529,5.DOI:10.3969/j.issn.1005-4847.2011.06.015
大鼠心肌梗死模型建立方法选择及心电图表现
Method selection for the establishment of myocardial infarction model and the electrocardiographic presentation in the rats
摘要
Abstract
Objective To explore the site of left coronary artery ligation on establishing myocardial infarction model and the electrocardiographic features in those rat models. Methods After the Sprague-Dawley(SD) rats were anaesthetized and put on chine-assisted respiration, an occluding suture was placed at one of two distinct sites along the left coronary artery. The suture was placed about 3 mm distal to the aortic root (high) and about 2 mm distal to the horizontal line of left atrial appendage tip (low) , respectively, and underwent the same procedure as the low suture group except left coronary artery ligation ( sham) , monitored by electrocardiography (ECG) before and after operation, respectively. At 4 weeks after operation they were sacrificed for histopathological analysis and measurement of the infarct area. Results The infarct area was about 55. 5% and 36. 2% , and the total survival rate was 13. 3% and 66. 7% in the high and low suture groups, re- spectively. There was no myocardial infarction and the survival rate was 100% in the sham operation group. ECG; QRS-T wave displayed an intersection "M" shape in the sham group and before left coronary artery ligation. The R wave and T wave fused into one large tent-like single wave after the left coronary artery ligation, and without visible ST segment. His-topathological changes of myocardial infarction were seen at 4 weeks after operation. Conclusions It is a novel method to establish myocardial infarct model that the suture is placed about 2 mm distal to the horizontal line of left atrial appendage tip, and all ECG showed no visible ST segment in the rats.关键词
SD大鼠/心肌梗死模型/心电图Key words
Rat/ Model, myocardial infarction/ Electrocardiogram分类
生物科学引用本文复制引用
王勇,李攀,高大中,殷跃辉,佘强,高翠菊,赵江龙,谭勇,唐永岗,陈运清..大鼠心肌梗死模型建立方法选择及心电图表现[J].中国实验动物学报,2011,19(6):525-529,5.基金项目
重庆市卫生局中医药科研资助项目(2006-B-25). （2006-B-25）
